With direct labeling, the probe DNA is synthesized with one or more modified dNTPs (the building block molecules of DNA) that have a fluorochrome chemical side group. By incorporating the fluorochrome directly into the probe, the fluorescent signal can be bound to the target in a single hybridization step. Direct labeled probes are simple to use because they require no signal amplification - this means that the fluorescent molecules attached to the DNA probe are of sufficient number that their fluorescent signals will be readily visible without any additional treatments immediately after hybridization. All Vysis
® FISH DNA probes are direct labeled.
The UroVysion Bladder Cancer Kit (UroVysion Kit) is designed to detect aneuploidy for chromosomes 3, 7, 17, and loss of the 9p21 locus via fluorescence in situ hybridization (FISH) in urine specimens from persons with hematuria suspected of having bladder cancer. Results from the UroVysion Kit are intended for use, in conjunction with and not in lieu of current standard diagnostic procedures, as an aid for initial diagnosis of bladder carcinoma in patients with hematuria and subsequent monitoring for tumor recurrence in patients previously diagnosed with bladder cancer.
