Hybridization
Hybridization is a process where a DNA sequence of interest is identified among other DNA sequences by pairing it with a complementary sequence used as a "probe". The process depends upon the biophysical properties of DNA nucleotide chains, which will unwind from a double helix at elevated temperatures and will rewind (pair or hybridize) with complementary sequences at lower temperatures.
The UroVysion Bladder Cancer Kit (UroVysion Kit) is designed to detect aneuploidy for chromosomes 3, 7, 17, and loss of the 9p21 locus via fluorescence in situ hybridization (FISH) in urine specimens from persons with hematuria suspected of having bladder cancer. Results from the UroVysion Kit are intended for use, in conjunction with and not in lieu of current standard diagnostic procedures, as an aid for initial diagnosis of bladder carcinoma in patients with hematuria and subsequent monitoring for tumor recurrence in patients previously diagnosed with bladder cancer.
